Pharmaceutical Microbiology Question Paper

Pharmaceutical Microbiology Question Paper With all Unit (B.pharm III semester) Here are a Selective questions of previous years questions papers collected by the different sources. this question papers are very helpful to all B. pharm III Sem students.

UNIT- 1 Pharmaceutical  Microbiology – Question – 10 Marks

• Classify bacteria on the basis of nutritional requirements and add a note on raw materials used for Preparation of culture
• Define and classify culture media. Mention salient feature of each media along with an
• Draw an ultra structure of typical bacteria. Write composition and functions of its
• Classify bacteria on the basis of morphological Add a note on composition and functions of Cell wall.
• Differentiate between gram positive and Gram negative cell Add a note on principle and Procedure of Gram’s staining technique.
• Describe bacterial growth Add a note on physical factors affecting growth of bacteria.
• Mention methods used for identification of Explain any four biochemical tests used for Identification of bacteria.
• What is pure culture? Enlist methods for isolation of pure culture? Describe any two industrially techniques of preserving
• Write about importance of microbial preservation technique. Write procedure, merit and demerit of any four preservation techniques.
• What is pure culture? Write in detail about isolation of pure.

Pharmaceutical microbiology Question- 07 Marks

• Write a note on raw materials used for preparation of culture
• Define and classify culture media with examples.
• Classify bacteria on the basis of morphological features.
• Differentiate between gram positive and Gram negative cell
• Write principle and procedure of Gram’s staining
• Write principle and procedure of Acid-fast
• Describe bacterial growth
• Explain IMVIC tests used for identification of
• Explain MR-VP tests used for identification of
• Write a note on cultivation of anaerobic
• Write about methods for maintenance of pure culture.
• Write about importance of microbial preservation
• Differentiate between prokaryotes and Eukaryotes
• Outline working of TEM and SEM
• Give the principle and main characteristic of phase contrast microscopy
• Explain the principle of Electron.

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Pharmaceutical microbiology Question- 02 Marks

• Differentiate between flagella and
• Differentiate between enrichment and selective
• Differentiate between log phase and decline
• Differentiate between acid fast and non acid fast
• Differentiate between bacteria and
• Differentiate between fungi and
• Mention reagents used for acid fast
• Mention role of each chemical used in gram’s staining.
• What is basal media? Give
• What is enriched media? Give
• What is differential media? Give example.
• What is the role of agar in culture media?
• What is selective media? Give
• What is pour plate method, write its
• Mention arrangement based classification of cocci.
• What is lag phase of growth?
• What is log phase of growth?
• What is stationary phase of growth?
• What is decline phase of growth?
• List out the different phases of growth of bacteria
• Write contributions of Antony Van
• Write contributions of Edward Jenner.
• Write contributions of Robert
• Write contributions of Louis Pasteur.
• Write contributions of Alexander
• Write four pharmaceutical uses of
• State Koch’s

UNIT-2- Pharmaceutical microbiology Question- 10 Marks

• Explain the principle, procedure, applications and demerits of sterilization using autoclave
• Explain the principle, procedure, applications and demerits of sterilization using hot air oven
• Explain the mechanism of action, procedure, applications and factors Sterilization using ethylene oxide.
• Explain the source, mechanism of sterilisation, merit, demerits and applications of sterilization using
• Explain principles involved in sterilisation by Add a note on its merits and demerits.

UNIT-2- Pharmaceutical microbiology Question – 07 Marks

• Explain the mechanism of sterilization and heat transfer by hot air
• Explain the principle involved in
• Write the procedure, merits and demerits of membrane filtration
• Write the procedure, merits and demerits of ethylene oxide sterilization
• Explain the factors affecting gaseous sterilization
• Mention indicators used for various sterilization methods
• Write the production, mechanism of action, demerits and applications of UV
• Explain MR-VP tests used for identification of
• Write the significance of various reagents used in Gram staining and Acid fast staining
• Write principle and procedure of Gram
• Write principle and procedure of acid-fast
• Explain IMVIC tests used for identification of
• Describe any two methods of viability
• Describe any two methods of total.

UNIT-2- Pharmaceutical microbiology Question- 02 mark’s

• Mention the demerits of ethylene oxide sterilization
• Write bio-indicators for thermal
• Write applications of uv
• What is Pasteurization? Mention methods
• Define
• Classify physical method of sterilization
• Explain the advantages of autoclaving over hot air sterilization.
• List out any four applications of Gamma
• Mention any four applications of autoclave.
• Mention any four applications of dry heat sterilization.
• Mention any four applications of gaseous sterilization.
• Mention any four applications of ethylene oxide sterilization

UNIT-3 – Microbiology Question- 10 Marks

• Classify Write the mechanism of action and uses of phenolic disinfectants.
• Explain different factors affecting
• Explain different methods for evaluation of bacteriostatic
• Discuss in detail about Redial Walker’s test.
• Enlist various methods of evaluation of bacteriostatic and
• Explain anyMethod of bacteriostatic disinfectant.
• Write classification, mechanism of action and uses of phenolic and aldehyde  disinfectants.

UNIT-3 – Microbiology Question- 07 Marks

• Write a note on cultivation of virus.
• Discuss about merits and demerits of viral cultivation
• Describe steps involved in replication of
• Write about classification of

UNIT-3 – Microbiology Question- 02 Marks

• Mention media for cultivation of
• Mention pharmaceutical uses of
• Write structure of typical
• What is mycelium?
• Why are virus described as obligate parasites?
• Give examples for disinfectants with viricidal activity
• Give two examples for alcoholic
• Differentiate between disinfection and
• Write the ideal properties of
• Define
• Mention the factors affecting disinfectant aactivity
• Difference between bacteriostatic and bactericidal agents.
• What is direct inoculation method?
• Write the significance of positive control in sterility
• What is direct inoculation method of sterility testing?
• Write the principle of sterility
• Write the principle of membrane filtration method of sterility testing.

UNIT – 4 – Microbiology  Question- 10 Marks

• Write in detail about the construction and design of aseptic
• What are the main sources of contamination of an aseptic room? How will you prevent  it?
• Describe construction and working of laminar air flow.
• Classify clean area according to British standard and US standards.
• What are the sources of contamination and their control in aseptic room?
• Describe the general procedure of antibiotic assay.
• Write briefly on different methods used for microbiological assay of antibiotics.
• Outline the process of assessment of new
• Explain principles involved in microbiological assay of Antibiotics.
• Explain principles involved in microbiological assay of vitamin B12.

• UNIT – 4 – Microbiology Question- 02 Marks

• What are Rodac plates?
• What is Grade 100?
• How will you validate HEPA filter?
• Write note on Grade B room
• Draw a flow diagram of an aseptic.

UNIT- 5 – Question- 10 Marks

1. Explain different factors affecting microbial spoilage of Pharmaceuticals
2. Define cell culture, give the merits, demerits and application of cell culture
3. Explain the different sources and types of microbial contamination of pharmaceuticals.
4. Write briefly the general procedure for cell culture.
5. How will you detect microbial contamination in pharmaceuticals
6. Write briefly on general requirements of cell culture
7. What are the methods used for the evaluation of microbial stability of formulations?
8. Differentiate between primary and established cell culture
9. Explain the evaluation of microbial stability of formulations.
10. Discuss about advantages and disadvantages of cell culture
11. Explain the preservation of pharmaceutical products using antimicrobial
12. Write the applications of cell cultures in pharmaceutical industry and research.

Unit 5 Question- 02 Marks

1. Mention microorganisms causing spoilage of pharmaceuticals.
2. Write the incubation conditions for cell culture.
3. What are the visible changes of microbial contaminations in pharmaceuticals
4. Mention any four major requirements for cell culture
5. How do you assess microbial contamination? Mention
6. Mention the media used in cell culture
7. List out different types of microbial contaminants in pharmaceutical.
8. What is secondary cell culture?
9. List out different types of spoilage in pharmaceutical.
10. What is primary cell culture?
11. List out sources of microbial contaminations in pharmaceutical.

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